Antibody was affinity purified using an epitope specific to TCF12 immobilized on solid support. The epitope recognized by A300-754A maps to a region between residue 1 and 50 of human Transcription Factor 12 using the numbering given in entry NP_996919.1 (GeneID 6938). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bHLHb20, class B basic helix-loop-helix protein 20, CRS3, DNA-binding protein HTF4, E-box-binding protein, HEB, helix-loop-helix transcription factor 4, HsT17266, HTF4, p64, TCF-12, transcription factor 12, transcription factor HTF-4
between 1 and 50
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human TCF12 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TCF12 antibody BL2270 (Cat. No. A300-754A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). TCF12 was also immunoprecipitated using rabbit anti-TCF12 antibody BL2271 (Cat. No. A300-755A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).