Antibody was affinity purified using an epitope specific to ZC3H13 immobilized on solid support. The epitope recognized by A300-748A maps to a region between residue 1175 and 1225 of human Zinc Finger CCCH-type Containing 13 using the numbering given in entry NP_055885.2 (GeneID 23091). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
KIAA0853, Xio, zinc finger CCCH domain-containing protein 13
between 1175 and 1225
2 - 8°C
Detection of human and mouse ZC3H13 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ZC3H13 antibody BL2287 (Cat. No. A300-748A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).