Antibody was affinity purified using an epitope specific to Cul4a immobilized on solid support. The epitope recognized by A300-739A maps to a region between residue 700 and the C-terminus (residue 759) of human Cullin 4A using the numbering given in entry NP_001008895.1 (GeneID 8451). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
between 700 and C-term
2 - 8°C
Detection of human and mouse Cul4a by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Cul4a antibody A300-739A (lot A300-739A-1) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Cul4a was also immunoprecipitated by rabbit anti-Cul4a antibody BL2390. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Detection of human and mouse Cul4a by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Cul4a (Cat. No. A300-739A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB