Antibody was affinity purified using an epitope specific to XLF immobilized on solid support. The epitope recognized by A300-730A maps to a region between residue 250 and the C-terminus (residue 299) of human XRCC4-Like Factor using the numbering given in entry NP_079058.1 (GeneID 79840). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
non-homologous end-joining factor 1, nonhomologous end-joining factor 1, protein cernunnos, XLF, XRCC4-like factor
between 250 and C-term
2 - 8°C
Detection of human XLF by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-XLF antibody BL3263 (Cat. No. A300-730A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). XLF was also immunoprecipitated using rabbit anti-XLF antibody BL3262 (Cat. No. A300-729A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Detection of human XLF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XLF (Cat. No. A300-730A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB