Antibody was affinity purified using an epitope specific to HDAC1 immobilized on solid support. The epitope recognized by A300-713A maps to region between residue 425 and the C-terminus (residue 482) of human Histone Deacetylase 1 using the numbering given in entry NP_004955.2 (GeneID 3065). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
GON-10, HD1, histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1, RPD3, RPD3L1
between 425 and C-term
2 - 8°C
Detection of human and mouse HDAC1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HDAC1 antibody BL2608 (Cat. No. A300-713A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). HDAC1 was also immunoprecipitated using rabbit anti-HDAC1 antibody BL2607 (Cat. No. A300-712A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of human and mouse HDAC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (top) and mouse squamous cell carcinoma (bottom). Antibody: Affinity purified rabbit anti-HDAC1 (Cat. No. A300-713A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:1,000 (0.2µg/ml). Detection: DAB