Antibody was affinity purified using an epitope specific to HDAC2 immobilized on solid support. The epitope recognized by A300-704A maps to the N-terminus of human Histone Deacetylase 2 using the numbering given in entry NP_001518.1 (GeneID 3066). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HD2, histone deacetylase 2, RPD3, transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1
2 - 8°C
Detection of human HDAC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded), from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC2 antibody BL2666 (Cat. No. A300-704A) used for IP at 3 µg/mg lysate. HDAC2 was also immunoprecipitated using rabbit anti-HDAC2 antibody BL2667 (Cat. No. A300-705A) at 3 µg/mg lysate. For blotting immunoprecipitated HDAC2, BL2667 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.