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Rabbit anti-PTEN Antibody, Affinity Purified

Catalogue number:
A300-701A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to PTEN immobilized on solid support. The epitope recognized by A300-701A maps to a region between residue 350 and the C-terminus (residue 403) of human phosphatase and tensin homolog using the numbering given in entry NP_000305.2 (GeneID 5728). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

10q23del, BZS, CWS1, DEC, GLM2, MHAM, mitochondrial phosphatase and tensin protein alpha, mitochondrial PTENalpha, MMAC1, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, mutated in multiple advanced cancers 1, Phosphatase and tensin homolog

Species Reactivity:

Human, Mouse

Immunogen:

between 350 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse PTEN by wes

Detection of human and mouse PTEN by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PTEN antibody A300-701A (lot A300-701A-1) used for WB at 0.04 µg (A) and 1 µg/ml (B) and used for immunoprecipitation at 3 µg/mg lysate (B). PTEN was also immunoprecipitated by rabbit anti-PTEN antibodies BL2788, BL2789, and A300-700A. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human PTEN by immunohistoch

Detection of human PTEN by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PTEN (Cat. No. A300-701A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB