Antibody was affinity purified using an epitope specific to PTEN immobilized on solid support. The epitope recognized by A300-701A maps to a region between residue 350 and the C-terminus (residue 403) of human phosphatase and tensin homolog using the numbering given in entry NP_000305.2 (GeneID 5728). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
10q23del, BZS, CWS1, DEC, GLM2, MHAM, mitochondrial phosphatase and tensin protein alpha, mitochondrial PTENalpha, MMAC1, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, mutated in multiple advanced cancers 1, Phosphatase and tensin homolog
between 350 and C-term
2 - 8°C
Detection of human and mouse PTEN by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PTEN antibody A300-701A (lot A300-701A-1) used for WB at 0.04 µg (A) and 1 µg/ml (B) and used for immunoprecipitation at 3 µg/mg lysate (B). PTEN was also immunoprecipitated by rabbit anti-PTEN antibodies BL2788, BL2789, and A300-700A. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of human PTEN by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PTEN (Cat. No. A300-701A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB