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Rabbit anti-hnRNP-U Antibody, Affinity Purified - A300-689A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-hnRNP-U Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-689A
Name:
Rabbit anti-hnRNP-U Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to hnRNP-U immobilized on solid support.The epitope recognized by A300-689A maps to a region between residue 450 and 500 of human Heterogeneous Nuclear Ribonucleoprotein U using the numbering given in entry NP_114032.1 (GeneID 3192).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
EIEE54, GRIP120, heterogeneous nuclear ribonucleoprotein U, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), hnRNP U, HNRNPU antisense RNA 1, HNRNPU-AS1, HNRPU, nuclear p120 ribonucleoprotein, p120, p120 nuclear protein, pp120, SA
Species Reactivity:
Human, Mouse
Immunogen:
Between 450 and 500
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse hnRNP-U by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-U antibody BL2763 (Cat. No. A300-689A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B). hnRNP-U was immunoprecipitated using rabbit anti-hnRNP-U antibody BL2765 (Cat. No. A300-690A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).

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Detection of human and mouse hnRNP-U by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-U (Cat. No. A300-689A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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