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Rabbit anti-CERT/GPBP Antibody, Affinity Purified

Catalogue number:
A300-668A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to CERT/GPBP immobilized on solid support. The epitope recognized by A300-668A maps to a region between residue 1 and 50 of human CERT/GPBP using the numbering given in entry NP_005704.1 (GeneID 10087). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

ceramide transfer protein, ceramide transporter, CERT, CERTL, collagen type IV alpha-3-binding protein, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein, Goodpasture antigen-binding protein, GPBP, hCERT, lipid-transfer protein CERTL, MRD34

Species Reactivity:

Human

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human CERT(GPBP) by western

Detection of human CERT(GPBP) by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CERT(GPBP) antibody BL2221 (Cat. No. A300-668A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CERT(GPBP) was also immunoprecipitated using rabbit anti-CERT(GPBP) antibody BL2222 (Cat. No. A300-669A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).