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Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£464.00 Estimated delivery Fri 5 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

surrounding serine 2

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Product Description:

Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human Phospho RNA Polymeras
Detection of human Phospho RNA Polymerase II (S2) by western blot. Samples: Whole cell lysate (50 and 15 µg) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A300-654A (lot A300-654A-3) was used for WB at 0.04 µg/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody A300-653A was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.
Detection of Phospho RNA Polymerase II (
Detection of Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. immunoprecipitates were either phosphatase treated (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A300-654A (lot A300-654A-3) used for IP at 5 µg per reaction and Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A used for IP at 5 µg per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S2) and total RNA Polymerase II, A300-654A was used at 0.1 µg/ml (upper panel) and A300-543A was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 75 seconds (lower).
Detection of human Phospho-RNA Polymeras
Detection of human Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) (Cat. No. A300-654A Lot3) used at a dilution of 1:1000 (0.2µg/ml). Detection: DAB
Detection of mouse Phospho-RNA Polymeras
Detection of mouse Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) (Cat. No. A300-654A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: DAB
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