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Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified

Catalogue number:
A300-654A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to Phospho RNA Polymerase II (S2) immobilized on solid support. Immunogen for A300-654A was a phosphorylated synthetic peptide, which represented a portion of human RNA Polymerase II (GeneID 5430) around serine 2 of the C-terminal repeat YSPTSPS. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, POLRA, polyme

Species Reactivity:

Human, Mouse

Immunogen:

surrounding serine 2

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Phospho RNA Polymeras

Detection of human Phospho RNA Polymerase II (S2) by western blot. Samples: Whole cell lysate (50 and 15 µg) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A300-654A (lot A300-654A-3) was used for WB at 0.04 µg/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody A300-653A was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.

Detection of Phospho RNA Polymerase II (

Detection of Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. immunoprecipitates were either phosphatase treated (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A300-654A (lot A300-654A-3) used for IP at 5 µg per reaction and Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A used for IP at 5 µg per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S2) and total RNA Polymerase II, A300-654A was used at 0.1 µg/ml (upper panel) and A300-543A was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 75 seconds (lower).

Detection of human Phospho-RNA Polymeras

Detection of human Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) (Cat. No. A300-654A Lot3) used at a dilution of 1:1000 (0.2µg/ml). Detection: DAB

Detection of mouse Phospho-RNA Polymeras

Detection of mouse Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) (Cat. No. A300-654A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: DAB