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  3. A300-654A
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Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified

Catalogue number:
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A300-654A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (200 µg/ml)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£513.00
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Estimated delivery Wed 31 Dec
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Applications:

IHC,IP,WB

Antibody Host:

Rabbit

Target:

RNA Polymerase II Phospho (S2)

Species Reactivity:

Human;Mouse

Immunogen:

Surrounding serine 2

Alternative Names:

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; POLR2; POLRA; polyme

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Product Description:

Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified - 100 µl (200 µg/ml)

Detection of human Phospho RNA Polymeras
Detection of human Phospho RNA Polymerase II (S2) by western blot. Samples: Whole cell lysate (50 and 15 µg) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A300-654A (lot A300-654A-3) was used for WB at 0.04 µg/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody A300-653A was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.
Detection of Phospho RNA Polymerase II (
Detection of Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. immunoprecipitates were either phosphatase treated (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A300-654A (lot A300-654A-3) used for IP at 5 µg per reaction and Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A used for IP at 5 µg per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S2) and total RNA Polymerase II, A300-654A was used at 0.1 µg/ml (upper panel) and A300-543A was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 75 seconds (lower).