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  3. A300-654A
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Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified

Catalogue number:
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A300-654A
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µg
Product is available in:
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£498.00
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Estimated delivery Wed 24 Sep
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Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Target:

RNA Polymerase II, Phospho (S2)

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Surrounding serine 2

Alternative Names:

RPBh1; RPOL2; RpIILS; RPB1; RNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; polymerase (RNA) II subunit A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; POLR2; hsRPB1; hRPB220; DNA-directed RNA polymerase III largest

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Product Description:

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide

Detection of human Phospho RNA Polymeras
Detection of human Phospho RNA Polymerase II (S2) by western blot. Samples: Whole cell lysate (50 and 15 µg) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A300-654A (lot A300-654A-3) was used for WB at 0.04 µg/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody A300-653A was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.
Detection of Phospho RNA Polymerase II (
Detection of Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. immunoprecipitates were either phosphatase treated (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A300-654A (lot A300-654A-3) used for IP at 5 µg per reaction and Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A used for IP at 5 µg per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S2) and total RNA Polymerase II, A300-654A was used at 0.1 µg/ml (upper panel) and A300-543A was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 75 seconds (lower).