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Rabbit anti-RNA Polymerase II Antibody, Affinity Purified

Catalogue number:
A300-653A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to RNA Polymerase II immobilized on solid support. The epitope recognized by A300-653A maps to the C-terminal repeat of human RNA Polymerase II (GeneID 5430). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, POLRA, polyme

Species Reactivity:

Human, Mouse

Immunogen:

C-terminal repeat

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse RNA Polymer

Detection of human and mouse RNA Polymerase II by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB), HeLa (H; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A (lot A300-653A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RNA Polymerase II by

Detection of human RNA Polymerase II by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HEK293T cells. Antibody: Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A (lot A300-653A-3) used for WB at 1 µg/ml and used for IP at 6 µg/mg lysate. RNA Polymerase II was also immunoprecipitated by a previous lot (lot A300-653A-2) of this antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.