Antibody was affinity purified using an epitope specific to RNA Polymerase II immobilized on solid support. The epitope recognized by A300-653A maps to the C-terminal repeat of human RNA Polymerase II (GeneID 5430). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, POLRA, polyme
2 - 8°C
Detection of human and mouse RNA Polymerase II by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB), HeLa (H; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A (lot A300-653A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human RNA Polymerase II by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HEK293T cells. Antibody: Affinity purified rabbit anti-RNA Polymerase II antibody A300-653A (lot A300-653A-3) used for WB at 1 µg/ml and used for IP at 6 µg/mg lysate. RNA Polymerase II was also immunoprecipitated by a previous lot (lot A300-653A-2) of this antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.