Antibody was affinity purified using an epitope specific to KIAA0310 immobilized on solid support. The epitope recognized by A300-648A maps to a region between residues 600 and 650 of human KIAA0310 using the numbering given in entry O15027 (GeneID 9919). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
KIAA0310, p250, protein SEC16 homolog A, protein transport protein Sec16A, SEC16 homolog A, SEC16L
between 600 and 650
2 - 8°C
Detection of human KIAA0310 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg for WB; 1 mg for IP) and HEK293T (50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-KIAA0310 antibody BL2467 (A300-648A) used for WB at 0.04 µg/ml and for IP at 3 µg/mg lysate. KIAA0310 was also immunoprecipitated by antibodies BL2468 and BL2469, which are to downstream epitopes on KIAA0301. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).