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Rabbit anti-CAP-D3 Antibody, Affinity Purified

Catalogue number:
A300-604A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to CAP-D3 immobilized on solid support. The epitope recognized by A300-604A maps to a region between residue 1450 and the C-terminus (residue 1507) of human Chromosome-Associated Protein D3 using the numbering given in entry NP_056076.1 (GeneID 23310). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

CAPD3, CAP-D3, condensin-2 complex subunit D3, hCAP-D3, hcp-6, hHCP-6, MCPH22, Non-SMC condensin II complex subunit D3

Species Reactivity:

Human

Immunogen:

between 1450 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human CAP-D3 by western blo

Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody A300-604A (lot A300-604A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CAP-D3 by western blo

Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody A300-604A (lot A300-604A-3) used for IP at 6 µg per reaction. CAP-D3 was also immunoprecipitated by rabbit anti-CAP-D3 antibody BL2943. For blotting immunoprecipitated CAP-D3, A300-604A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.