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Rabbit anti-GNL3 Antibody, Affinity Purified - A300-600A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-GNL3 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-600A
Name:
Rabbit anti-GNL3 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to GNL3 immobilized on solid support. The epitope recognized by A300-600A maps to a region between residue 500 and the C-terminus (residue 549) of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) using the numbering given in entry NP_055181.3 (GeneID 26354).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
C77032, E2IG3, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide binding protein-like 3 (nucleolar), guanine nucleotide-binding protein-like 3, NNP47, novel nucleolar protein 47, NS, nucleolar GTP-binding protein
Species Reactivity:
Human, Mouse
Immunogen:
Between 500 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human GNL3 by western blot. Samples:Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody A300-600A (lot A300-600A-3) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 µg) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody A300-600A (lot A300-600A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human GNL3 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GNL3 antibody A300-600A (lot A300-600A-3) used for IP at 6 µg per reaction. GNL3 was also immunoprecipitated by a previous lot of this antibody (lot A300-600A-2). For blotting immunoprecipitated GNL3, A300-600A was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human GNL3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (Cat. No. A300-600A lot 3) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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Detection of mouse GNL3 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (Cat. No. A300-600A lot 3) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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