Antibody was affinity purified using an epitope specific to BRIP1/BACH1 immobilized on solid support. The epitope recognized by A300-561A maps to a region between residue 1200 and the C-terminus (residue 1249) of human Brca1 Interacting Protein C-terminal Helicase 1 using the numbering given in entry NP_114432.1 (GeneID 83990). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase BRIP1, BACH1, BRCA1/BRCA2-associated helicase 1, BRCA1-associated C-terminal helicase 1, BRCA1-binding helicase-like protein BACH1, BRCA1-interacting protein 1, BRCA1-interacting protein C-terminal helicase 1, FANCJ, Fanconi ane
between 1200 and C-term
2 - 8°C
Detection of human BRIP1(BACH1) by western blot and immunoprecipitation. expndtw-6 Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP) and HEK293T (T; 50 µg for WB) cells. Antibody: Affinity purified rabbit anti-BRIP1(BACH1) antibody BL2600 (Cat. No. A300-561A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate. BRIP1(BACH1) was also immunoprecipitated using rabbit anti-BRIP1(BACH1) antibody BL2599 (Cat. No. A300-561A), which is to an upstream epitope, at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).