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Rabbit anti-ZBTB7/FBI-1 Antibody, Affinity Purified

Catalogue number:
A300-548A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to ZBTB7/FBI-1 immobilized on solid support. The epitope recognized by A300-548A maps to a region between residues 200 and 250 of human ZBTB7 (Factor Binding IST protein 1) using the numbering given in entry NP_056982.1 (GeneID 51341). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

factor binding IST protein 1, factor that binds to inducer of short transcripts protein 1, FBI1, FBI-1, HIV-1 1st-binding protein 1, HIV-1 inducer of short transcripts binding protein, leukemia/lymphoma-related factor, LRF, lymphoma related factor, POK er

Species Reactivity:

Human, Mouse

Immunogen:

between 200 and 250

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human ZBTB7/FBI-1 by wester

Detection of human ZBTB7/FBI-1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody A300-548A (lot A300-548A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse ZBTB7/FBI-1 by wester

Detection of mouse ZBTB7/FBI-1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody A300-548A (lot A300-548A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human ZBTB7/FBI-1 by wester

Detection of human ZBTB7/FBI-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody A300-548A (lot A300-548A-3) used for IP at 3 µg per reaction. ZBTB7/FBI-1 was also immunoprecipitated by a previous lot of this antibody (lot A300-548A-2) and rabbit anti-ZBTB7/FBI-1 antibody A300-549A. For blotting immunoprecipitated ZBTB7/FBI-1, A300-548A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.