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Rabbit anti-DDX19 Antibody, Affinity Purified - A300-547A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DDX19 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-547A
Name:
Rabbit anti-DDX19 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DDX19 immobilized on solid support. The epitope recognized by A300-547A maps to a region between residue 450 and the C-terminus (residue 479) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B using the numbering given in entry NP_009173.1 (GeneID 11269). The epitope is highly conserved with DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed.Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ATP-dependent RNA helicase DDX19, ATP-dependent RNA helicase DDX19B, DBP5, DDX19, DEAD (Asp-Glu-Ala-As) box polypeptide 19B, DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, DEAD/H (Asp-Glu-Ala-Asp/His) box po
Species Reactivity:
Human, Mouse
Immunogen:
Between 450 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse DDX19 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg) and NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-DDX19 antibody (Cat. No. A300-547A) used at 0.04 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human DDX19 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX19 (Cat. No. A300-547A) used at a dilution of 1:200 ( 1µg/ml). Detection: DAB

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