Antibody was affinity purified using an epitope specific to DDX19 immobilized on solid support. The epitope recognized by A300-547A maps to a region between residue 450 and the C-terminus (residue 479) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B using the numbering given in entry NP_009173.1 (GeneID 11269). The epitope is highly conserved with DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DDX19, ATP-dependent RNA helicase DDX19B, DBP5, DDX19, DEAD (Asp-Glu-Ala-As) box polypeptide 19B, DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, DEAD/H (Asp-Glu-Ala-Asp/His) box po
between 450 and C-term
2 - 8°C
Detection of human and mouse DDX19 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg) and NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-DDX19 antibody (Cat. No. A300-547A) used at 0.04 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human DDX19 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX19 (Cat. No. A300-547A) used at a dilution of 1:200 ( 1µg/ml). Detection: DAB