Antibody was affinity purified using an epitope specific to JIK/TAOK3 immobilized on solid support. The epitope recognized by A300-536A maps to a region between residue 850 and the C-terminus (residue 898) of human JNK/SAPK-Inhibitory Kinase (TAO kinase 3) using the numbering given in entry NP_057365.2 (GeneID 51347). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CTCL-associated antigen HD-CL-09, cutaneous T-cell lymphoma-associated antigen HD-CL-09, dendritic cell-derived protein kinase, DPK, hKFC-A, JIK, JNK/SAPK-inhibitory kinase, jun kinase-inhibitory kinase, kinase from chicken homolog A, MAP3K18, serine/thre
between 850 and C-term
2 - 8°C
Detection of human and mouse JIK/TAOK3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JIK/TAOK3 antibody A300-536A (lot A300-536A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human JIK/TAOK3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JIK/TAOK3 antibody A300-536A (lot A300-536A- 3 ) used for IP at 6 µg per reaction. JIK/TAOK3 was also immunoprecipitated by a previous lot of this antibody (A300-536A-1) and rabbit anti-JIK/TAOK3 antibody A300-535A-1. For blotting immunoprecipitated JIK/TAOK3, A300-536A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.