Antibody was affinity purified using an epitope specific to UBAP2L/NICE4 immobilized on solid support. The epitope recognized by A300-534A maps to a region between residue 1025 and the C-terminus (residue 1087) of human Ubiquitin Associated Protein 2-Like using the numbering given in entry AAH03170.1 (GeneID 9898). The epitope is not found in the shorter entry BAA09765.1. The more quickly migrating protein detected by using A300-533A (but not detected by A300-534A) is believed to be the shorter from of UBAP2L/NICE4. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
NICE4, NICE-4, protein NICE-4, ubiquitin-associated protein 2-like
between 1025 and C-term
2 - 8°C
Detection of human UBAP2L/NICE-4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBAP2L/NICE-4 antibody A300-534A (lot A300-534A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human UBAP2L/NICE4 by western blot. Samples: Whole cell lysate (40 µg) from HeLa cells mock treated, treated with a control siRNA, or treated with siRNA for UBAP2L/NICE4. Antibody: Affinity purified rabbit anti-UBAP2L/NICE4 antibody (Cat. No. A300-534A) used at 0.03 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.