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Rabbit anti-UBAP2L/NICE4 Antibody, Affinity Purified

Catalogue number:
A300-533A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to UBAP2L/NICE4 immobilized on solid support. The epitope recognized by A300-533A maps to a region between residues 400 and 450 of human Ubiquitin Associated Protein 2-Like using the numbering given in entry AAH03170.1 (GeneID 9898). The epitope is also found in the shorter entry BAA09765.1. The more quickly migrating protein detected by using A300-533A (but not detected by A300-534A) is believed to be the shorter from of UBAP2L/NICE4. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

NICE4, NICE-4, protein NICE-4, ubiquitin-associated protein 2-like

Species Reactivity:

Human

Immunogen:

between 400 and 450

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human UBAP2L/NICE4 by weste

Detection of human UBAP2L/NICE4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBAP2L/NICE4 antibody A300-533A (lot A300-533A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human UBAP2L/NICE4 by weste

Detection of human UBAP2L/NICE4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UBAP2L/NICE4 antibody A300-533A (lot A300-533A-2) used for IP at 6 µg per reaction. UBAP2L/NICE4 was also immunoprecipitated by rabbit anti-UBAP2L/NICE4 antibody A300-534A. For blotting immunoprecipitated UBAP2L/NICE4, A300-533A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.