Antibody was affinity purified using an epitope specific to UBAP2L/NICE4 immobilized on solid support. The epitope recognized by A300-533A maps to a region between residues 400 and 450 of human Ubiquitin Associated Protein 2-Like using the numbering given in entry AAH03170.1 (GeneID 9898). The epitope is also found in the shorter entry BAA09765.1. The more quickly migrating protein detected by using A300-533A (but not detected by A300-534A) is believed to be the shorter from of UBAP2L/NICE4. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
NICE4, NICE-4, protein NICE-4, ubiquitin-associated protein 2-like
between 400 and 450
2 - 8°C
Detection of human UBAP2L/NICE4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBAP2L/NICE4 antibody A300-533A (lot A300-533A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human UBAP2L/NICE4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UBAP2L/NICE4 antibody A300-533A (lot A300-533A-2) used for IP at 6 µg per reaction. UBAP2L/NICE4 was also immunoprecipitated by rabbit anti-UBAP2L/NICE4 antibody A300-534A. For blotting immunoprecipitated UBAP2L/NICE4, A300-533A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.