Antibody was affinity purified using an epitope specific to eIF4G1/eIF4GI immobilized on solid support. The epitope recognized by A300-502A maps to a region between residues 575 and 625 of human Eukaryotic Initiation Factor 4 Gamma 1 using the numbering given in entry NP_886553.2 (GeneID 1981). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
EIF4F, EIF4G, eIF-4G 1, EIF-4G1, EIF4-gamma, eIF-4-gamma 1, EIF4GI, eucaryotic translation initiation factor 4G, eukaryotic translation initiation factor 4 gamma 1, P220, PARK18
between 575 and 625
2 - 8°C
Detection of human eiF4G1/eiF4GI by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody A300-502A (lot A300-502A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human eiF4G1/eiF4GI by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody A300-502A (lot A300-502A-2) used for IP at 6 µg per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit anti-eiF4G1/eiF4GI antibody A301-776A. For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit anti-eiF4G1/eiF4GI antibody A301-774A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.