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Rabbit anti-Estrogen Receptor Alpha Antibody, Affinity Purified

Catalogue number:
A300-498A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Estrogen Receptor Alpha immobilized on solid support. The epitope recognized by A300-498A maps to a region between residue 550 and the C-terminus (residue 595) of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

E2 receptor alpha, ER, Era, ER-alpha, ESR, ESRA, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2, ESTRR, NR3A1, nuclear receptor subfamily 3 group A member 1,

Species Reactivity:

Human

Immunogen:

between 550 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Estrogen Receptor Alp

Detection of human Estrogen Receptor Alpha by western blot. Samples: Whole cell lysate (50 µg) from MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Estrogen Receptor Alpha antibody A300-498A (lot A300-498A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Estrogen Receptor Alp

Detection of human Estrogen Receptor Alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Estrogen Receptor Alpha antibody A300-498A (lot A300-498A-3) used for IP at 6 µg per reaction. Estrogen Receptor Alpha was also immunoprecipitated by previous lot of this antibody A300-498A-2. For blotting immunoprecipitated Estrogen Receptor Alpha, A300-498A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.