Rabbit anti-Estrogen Receptor Alpha Antibody, Affinity Purified - A300-498A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Estrogen Receptor Alpha immobilized on solid support.The epitope recognized by A300-498A maps to a region between residue 550 and the C-terminus (residue 595) of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human Estrogen Receptor Alpha by western blot. Samples: Whole cell lysate (50 µg) from MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Estrogen Receptor Alpha antibody A300-498A (lot A300-498A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Estrogen Receptor Alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Estrogen Receptor Alpha antibody A300-498A (lot A300-498A-3) used for IP at 6 µg per reaction. Estrogen Receptor Alpha was also immunoprecipitated by previous lot of this antibody A300-498A-2. For blotting immunoprecipitated Estrogen Receptor Alpha, A300-498A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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