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Rabbit anti-Estrogen Receptor Alpha Antibody, Affinity Purified

Catalogue number:
A300-497A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Estrogen Receptor Alpha immobilized on solid support. The epitope recognized by A300-497A maps to a region between residues 525 and 575 of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Product Type:

Antibodies

Alternative Names:

E2 receptor alpha, ER, Era, ER-alpha, ESR, ESRA, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2, ESTRR, NR3A1, nuclear receptor subfamily 3 group A member 1,

Species Reactivity:

Human

Immunogen:

between 525 and 575

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Estrogen Receptor Alp

Detection of human Estrogen Receptor Alpha by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-ER alpha antibodies BL2051 (Cat. No. A300-495A), BL2052 (Cat. No. A300-496A), BL2053 (Cat. No. A300-497A) and BL2054 (Cat. No. A300-498A) each used at 3 µg/mg lysate for IP. Estrogen receptor alpha was blotted using BL2054 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.