Antibody was affinity purified using an epitope specific to Estrogen Receptor Alpha immobilized on solid support. The epitope recognized by A300-497A maps to a region between residues 525 and 575 of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E2 receptor alpha, ER, Era, ER-alpha, ESR, ESRA, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2, ESTRR, NR3A1, nuclear receptor subfamily 3 group A member 1,
between 525 and 575
2 - 8°C
Detection of human Estrogen Receptor Alpha by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-ER alpha antibodies BL2051 (Cat. No. A300-495A), BL2052 (Cat. No. A300-496A), BL2053 (Cat. No. A300-497A) and BL2054 (Cat. No. A300-498A) each used at 3 µg/mg lysate for IP. Estrogen receptor alpha was blotted using BL2054 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.