Antibody was affinity purified using an epitope specific to Estrogen Receptor Alpha immobilized on solid support. The epitope recognized by A300-495A maps to a region between residues 1 and 50 of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E2 receptor alpha, ER, Era, ER-alpha, ESR, ESRA, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2, ESTRR, NR3A1, nuclear receptor subfamily 3 group A member 1,
between 1 and 50
2 - 8°C
Detection of human Estrogen Receptor Alpha by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-ER alpha antibodies A300-495A (lot A300-495A-1), BL2052, A300-497A, and A300-498A each used at 3 µg/mg lysate for IP. Estrogen receptor alpha was blotted using A300-498A at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Localization of Estrogen Receptor Alpha Binding Sites by ChIP-sequencing. Chromatin from treated MCF-7 cells was immunoprecipitated with anti-Estrogen Receptor Alpha antibody A300-495A and analyzed by DNA sequencing. The figure illustrates the peak distribution of Estrogen Receptor Alpha binding within a 250 Kb region of chromosome 21 as detected using anti-Estrogen Receptor Alpha antibody A300-495A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.