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Rabbit anti-Estrogen Receptor Alpha Antibody, Affinity Purified

Catalogue number:
A300-495A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to Estrogen Receptor Alpha immobilized on solid support. The epitope recognized by A300-495A maps to a region between residues 1 and 50 of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP, IHC, ChIP-Seq
Product Type:

Antibodies

Alternative Names:

E2 receptor alpha, ER, Era, ER-alpha, ESR, ESRA, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2, ESTRR, NR3A1, nuclear receptor subfamily 3 group A member 1,

Species Reactivity:

Human

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Estrogen Receptor Alp

Detection of human Estrogen Receptor Alpha by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-ER alpha antibodies A300-495A (lot A300-495A-1), BL2052, A300-497A, and A300-498A each used at 3 µg/mg lysate for IP. Estrogen receptor alpha was blotted using A300-498A at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Estrogen Receptor Alp

Detection of human Estrogen Receptor Alpha by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-Estrogen Receptor Alpha (Cat. No. A300-495A) used at a dilution of 1:2,500 (0.4µg/ml). Detection: DAB

Localization of Estrogen Receptor Alpha

Localization of Estrogen Receptor Alpha Binding Sites by ChIP-sequencing. Chromatin from treated MCF-7 cells was immunoprecipitated with anti-Estrogen Receptor Alpha antibody A300-495A and analyzed by DNA sequencing. The figure illustrates the peak distribution of Estrogen Receptor Alpha binding within a 250 Kb region of chromosome 21 as detected using anti-Estrogen Receptor Alpha antibody A300-495A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.