Antibody was affinity purified using an epitope specific to CtIP (RBBP8) immobilized on solid support. The epitope recognized by A300-488A maps to a region between residue 850 and the C-terminus (residue 897) of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8) using the numbering given in entry NP_002885.1 (GeneID 5932). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
COM1, CTBP-interacting protein, CTIP, DNA endonuclease RBBP8, JWDS, RBBP-8, retinoblastoma binding protein 8, Retinoblastoma-binding protein 8, Retinoblastoma-interacting protein and myosin-like, RIM, SAE2, SCKL2, sporulation in the absence of SPO11 prote
between 850 and C-term
2 - 8°C
Detection of human and mouse CtIP by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CtIP antibody A300-488A (lot A300-488A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CtIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CtIP antibody A300-488A (lot A300-488A-5) used for IP at 3 µg per reaction. CtIP was also immunoprecipitated by rabbit anti-CtIP antibody A300-487A. For blotting immunoprecipitated CtIP, A300-488A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.