Antibody was affinity purified using an epitope specific to LATS1 (WARTS) immobilized on solid support. The epitope recognized by A300-477A maps to a region between residues 50 and 100 of human Large Tumor Suppressor homolog 1 using the numbering given in SwissProt entry O95835 (GeneID 9113). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
h-warts, large tumor suppressor homolog 1, LATS (large tumor suppressor, Drosophila) homolog 1, LATS, large tumor suppressor, homolog 1, serine/threonine-protein kinase LATS1, WARTS, WARTS protein kinase, wts
between 50 and 100
2 - 8°C
Detection of human LATS1 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 1/4 of IP loaded/lane) from HeLa Cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody BL2210 (Cat. No. A300-477A) used at 0.02 (A) and 0.1 (B) µg/ml for WB and at 3 µg/mg lysate for IP (B). LATS1 was also immunoprecipitated with LATS1 antibodies BL2211 and BL2212 (Cat. No. A300-478A) using 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).
Detection of mouse LATS1 by western blot. Samples: Whole cell lysate (50 µg) from CT26.WT and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody A300-477A (lot A300-477A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.