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Rabbit anti-CSE1 Antibody, Affinity Purified - A300-472A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-CSE1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-472A
Name:
Rabbit anti-CSE1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to CSE1 immobilized on solid support. The epitope recognized by A300-472A maps to a region between residues 1 and 50 of human Chromosome Segregation-1 using the numbering given in entry NP_001307.2 (GeneID 1434).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
CAS, cellular apoptosis susceptibility protein, chromosome segregation 1-like protein, CSE1, CSE1 chromosome segregation 1-like, epididymis secretory sperm binding protein, exp2, exportin-2, importin-alpha re-exporter, XPO2
Species Reactivity:
Human, Mouse
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse CSE1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody A300-472A (lot A300-472A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human CSE1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody A300-472A (lot A300-472A-2) used for IP at 6 µg per reaction. CSE1 was also immunoprecipitated by a previous lot of this antibody (A300-472A-1) and rabbit anti-CSE1 antibody BL1977. For blotting immunoprecipitated CSE1, A300-472A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human CSE1 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-CSE1 (Cat. No. A300-472A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB

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Detection of mouse CSE1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CSE1 (Cat. No. A300-472A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB

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