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Rabbit anti-TPX2 Antibody, Affinity Purified - A300-429A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-TPX2 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-429A
Name:
Rabbit anti-TPX2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to TPX2 immobilized on solid support. The epitope recognized by A300-429A maps to a region between residues 150 and 200 of human TPX2 using the numbering given in Swiss-Prot entry Q9ULW0 (GeneID 22974).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, ICC-IF

Product Type:
Antibodies
Alternative Names:
C20orf1, C20orf2, differentially expressed in cancerous and non-cancerous lung cells 2, differentially expressed in lung cells, DIL2, DIL-2, FLS353, GD:C20orf1, HCA519, HCTP4, hepatocellular carcinoma-associated antigen 519, hepatocellular carcinoma-assoc
Species Reactivity:
Human
Immunogen:
Between 150 and 200
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human TPX2 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL; 5, 15, 50 µg for A; 50 µg for Input and 1 mg for IP in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-TPX2 antibody BL1677 (Cat. No. A300-429A) used at 0.1 µg/ml for WB (A & B) and at 0.4 µg/mg lysate for IP (B). TPX2 was also immunoprecipitated using rabbit anti-TPX2 antibody BL1679 (Cat. No. A300-430A) at 0.4 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 1 minute.

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Detection of human TPX2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cell. Antibody: Affinity purified rabbit anti- TPX2 (Cat. No.A300-429A Lot1) used at a dilution of 1:400 (2.5µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100

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