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Rabbit anti-ASC2 Antibody, Affinity Purified - A300-410A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ASC2 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-410A
Name:
Rabbit anti-ASC2 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to ASC2 immobilized on solid support.The epitope recognized by A300-410A maps to a region between residues 1050 and 1100 of human Activating Signal Cointegrator-2 using the numbering given in Swiss-Prot entry Q14686 (GeneID 23054).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
Activating signal cointegrator 2, activating signal cointegrator-2, AIB3, amplified in breast cancer protein 3, amplified in breast cancer-3 protein, ASC2, ASC-2, cancer-amplified transcriptional coactivator ASC-2, NRC, NRC RAP250, nuclear receptor coacti
Species Reactivity:
Human
Immunogen:
Between 1050 and 1100
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human ASC2 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody A300-410A (lot A300-410A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human ASC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody A300-410A (lot A300-410A-2) used for IP at 6 µg per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody (lot A300-410A-1) and rabbit anti-ASC2 antibody A300-411A. For blotting immunoprecipitated ASC2, A300-410A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human ASC2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 (Cat. No. A300-410A Lot2) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB

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