Antibody was affinity purified using an epitope specific to ASC2 immobilized on solid support. The epitope recognized by A300-410A maps to a region between residues 1050 and 1100 of human Activating Signal Cointegrator-2 using the numbering given in Swiss-Prot entry Q14686 (GeneID 23054). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Activating signal cointegrator 2, activating signal cointegrator-2, AIB3, amplified in breast cancer protein 3, amplified in breast cancer-3 protein, ASC2, ASC-2, cancer-amplified transcriptional coactivator ASC-2, NRC, NRC RAP250, nuclear receptor coacti
between 1050 and 1100
2 - 8°C
Detection of human ASC2 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody A300-410A (lot A300-410A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human ASC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody A300-410A (lot A300-410A-2) used for IP at 6 µg per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody (lot A300-410A-1) and rabbit anti-ASC2 antibody A300-411A. For blotting immunoprecipitated ASC2, A300-410A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.