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Rabbit anti-Nanog Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Wed 3 Apr
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

Nanog

Species Reactivity:

Mouse

Antibody Type:

Polyclonal

Immunogen:

between 1 and 50

Alternative Names:

ecat4; homeobox transcription factor Nanog; homeobox protein NANOG; ENK; early embryo specific expression NK-type homeobox protein; early embryo specific expression NK family; 2410002E02Rik; ES cell-associated protein 4

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Product Description:

Rabbit anti-Nanog Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of mouse Nanog by western blot
Detection of mouse Nanog by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-397A (lot A300-397A-3) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of mouse Nanog by western blot
Detection of mouse Nanog by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-397A (lot A300-397A-3) used for IP at 6 µg per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody A300-398A. For blotting immunoprecipitated Nanog, A300-397A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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