Tel: +44 (0)1223 316 855
life science research products, biological research products, biotechnology
close ...


Supplier:
Product types:

Rabbit anti-Nanog Antibody, Affinity Purified - A300-397A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Nanog Antibody, Affinity Purified
print page
Size: 100 µg
£302.00
GBP | Euro | USD
Add to basket
Catalogue number:
A300-397A
Name:
Rabbit anti-Nanog Antibody, Affinity Purified
Supplier:
Availability:

Antibody was affinity purified using an epitope specific to Nanog immobilized on solid support. The epitope recognized by A300-397A maps to a region between residues 1 and 50 of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
2410002E02Rik, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, ecat4, ENK, ES cell-associated protein 4, homeobox protein NANOG, homeobox transcription factor Nanog
Species Reactivity:
Mouse
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
thumbnail

Detection of mouse Nanog by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-397A (lot A300-397A-3) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

thumbnail

Detection of mouse Nanog by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-397A (lot A300-397A-3) used for IP at 6 µg per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody A300-398A. For blotting immunoprecipitated Nanog, A300-397A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Contact Our Friendly Technical Team