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Rabbit anti-Nanog Antibody, Affinity Purified

Catalogue number:
A300-397A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to Nanog immobilized on solid support. The epitope recognized by A300-397A maps to a region between residues 1 and 50 of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

2410002E02Rik, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, ecat4, ENK, ES cell-associated protein 4, homeobox protein NANOG, homeobox transcription factor Nanog

Species Reactivity:

Mouse

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of mouse Nanog by western blot

Detection of mouse Nanog by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-397A (lot A300-397A-3) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse Nanog by western blot

Detection of mouse Nanog by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-397A (lot A300-397A-3) used for IP at 6 µg per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody A300-398A. For blotting immunoprecipitated Nanog, A300-397A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.