Antibody was affinity purified using an epitope specific to DHX15 immobilized on solid support. The epitope recognized by A300-389A maps to a region between residues 350 and 400 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase #46, DBP1, DDX15, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15, DEAD/H box-15, DEAH (Asp-Glu-Ala-His) box helicase 15, DEAH (Asp-Glu-Ala-His) box polypeptide 15, DEAH box protein 15, HRH2, pre-mRNA-splicing factor ATP-depende
between 350 and 400
2 - 8°C
Detection of human DHX15 by western blot and immunoprecipitation. Samples: Whole cell lysate (CL; 60 µg for WB, 1 mg for IP) from HEK293T cells. Antibodies: Affinity purified rabbit anti-DHX15 antibody BL1627 (Cat. No. A300-389A) used at 0.1 µg/ml for WB and at 1 µg/mg lysate for IP, which did not precipitate DHX15. Successful IP of DHX15 was accomplished using affinity purified rabbit anti-DHX15 antibody BL1628 (Cat. No. A300-390A) at 1 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 15 seconds.
Detection of human DHX15 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DHX15 (Cat. No. A300-389A) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB