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Rabbit anti-Bcl11a Antibody, Affinity Purified - A300-382A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Bcl11a Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-382A
Name:
Rabbit anti-Bcl11a Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Bcl11a immobilized on solid support. The epitope recognized by A300-382A maps to a region between residue 775 and the C-terminus (residue 835) of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
B-cell CLL/lymphoma 11A, B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2, B-cell lymphoma/leukemia 11A, BCL-11A, BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1, BCL11A-L, BCL11a-M, BCL11A-S, BCL11A-XL, C2H2-type zinc finger protein
Species Reactivity:
Human, Mouse
Immunogen:
Between 775 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Bcl11a by western blot and immunoprecipitation. Samples: Whole cell lysate from Ramos (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Bcl11a antibody A300-382A (lot A300-382A-2) used for WB at 1 µg/ml (A and B) and used for IP at 6 µg/mg lysate. Bcl11a was also immunoprecipitated by a prior lot (lot A300-382A-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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Detection of mouse Bcl11a by western blot. Samples: Whole cell lysate (50 µg) from L1210, TCMK-1, and 4T1 cells. Antibodies: Affinity purified rabbit anti-Bcl11a antibody A300-382A (lot A300-382A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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