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Rabbit anti-Bcl11a Antibody, Affinity Purified

Catalogue number:
A300-381A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to Bcl11a immobilized on solid support. The epitope recognized by A300-381A maps to a region between residues 450 and 500 of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

B-cell CLL/lymphoma 11A, B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2, B-cell lymphoma/leukemia 11A, BCL-11A, BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1, BCL11A-L, BCL11a-M, BCL11A-S, BCL11A-XL, C2H2-type zinc finger protein

Species Reactivity:

Human

Immunogen:

between 450 and 500

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Bcl11a by western blo

Detection of human Bcl11a by western blot. Samples: Whole cell lysate from Ramos (50 and 15 µg), HeLa (50 µg), and HEK293T (50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11a antibody A300-381A (lot A300-381A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Bcl11a by western blo

Detection of human Bcl11a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Bcl11a antibody A300-381A (lot A300-381A-2) used for IP at 6 µg per reaction. Bcl11a was also immunoprecipitated by rabbit anti-Bcl11a antibody A300-381A-1. For blotting immunoprecipitated Bcl11a, A300-381A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.