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High Quality Recombinant Rabbit Monoclonal Antibodies
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Rabbit anti-PBEF Antibody, Affinity Purified - A300-372A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PBEF Antibody, Affinity Purified
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Size: 100 µg
£334.00
GBP | Euro | USD
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Catalogue number:
A300-372A
Name:
Rabbit anti-PBEF Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to PBEF immobilized on solid support. The epitope recognized by A300-372A maps to a region between residues 400 and 450 of human pre-B-cell colony enhancing factor 1 using the numbering given in Swiss-Prot entry P43490 (GeneID 10135).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IHC

Product Type:
Antibodies
Alternative Names:
1110035O14Rik, NAmPRTase, Nampt, nicotinamide phosphoribosyltransferase, PBEF, PBEF1, pre-B cell-enhancing factor, pre-B-cell colony-enhancing factor 1, VF, VISFATIN
Species Reactivity:
Human, Mouse
Immunogen:
Between 400 and 450
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse PBEF by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody A300-372A (lot A300-372A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human PBEF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBEF antibody A300-372A (lot A300-372A-5) used for IP at 6 µg per reaction. PBEF was also immunoprecipitated by rabbit anti-PBEF antibodies A300-778A and A300-779A. For blotting immunoprecipitated PBEF, A300-372A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human PBEFby immunohistochemistry. Sample: FFPE section of human placental fetal membrane. Antibody: Affinity purified rabbit anti-PBEF (Cat. No. A300-372A Lot 5) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB

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