Antibody was affinity purified using an epitope specific to Caper immobilized on solid support. The epitope recognized by A300-353A maps to a region between residues 480 and the C-terminus (residue 530) of human Caper, Coactivator of activating protein-1 and estrogen receptors using the numbering given in SwissProt entry Q14498 (GeneID 9584). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAPER, CAPER alpha, CAPERalpha, coactivator of activating protein-1 and estrogen receptors, epididymis secretory sperm binding protein, FSAP59, functional spliceosome-associated protein 59, HCC1, hepatocellular carcinoma protein 1, RNA-binding motif prote
between 480 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Caper by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caper antibody A300-353A (lot A300-353A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.