Rabbit anti-p14ARF Antibody, Affinity Purified - A300-342A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to p14ARF immobilized on solid support. The epitope recognized by A300-342A maps to a region between residue 125 and the C-terminus (residue 173) of human p14ARF using the numbering given in entry NP_478102.1 (GeneID 1029). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IHC
Detection of human p14ARF by western blot. Samples: Whole cell lysate (25 µg) from BT549 (positive control), U2OS (negative control) and p14ARF transfected IMR90 cells. Antibody: Affinity purified rabbit anti-p14ARF (Cat. No. A300-342A) used for WB at 1 µg/ml. Detection: Chemiluminescence.
Detection of human p14ARF by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF (Cat. No. A300-342A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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