Antibody was affinity purified using an epitope specific to p14ARF immobilized on solid support. The epitope recognized by A300-342A maps to a region between residue 125 and the C-terminus (residue 173) of human p14ARF using the numbering given in entry NP_478102.1 (GeneID 1029). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
alternative reading frame, ARF, CDK4 inhibitor p16-INK4, CDK4I, CDKN2, cell cycle negative regulator beta, CMM2, cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK
between 82 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide