Antibody was affinity purified using an epitope specific to p14ARF immobilized on solid support. The epitope recognized by A300-342A maps to a region between residue 125 and the C-terminus (residue 173) of human p14ARF using the numbering given in entry NP_478102.1 (GeneID 1029). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
alternative reading frame, ARF, CDK4 inhibitor p16-INK4, CDK4I, CDKN2, cell cycle negative regulator beta, CMM2, cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK
between 125 and C-term
2 - 8°C
Detection of human p14ARF by western blot. Samples: Whole cell lysate (25 µg) from BT549 (positive control), U2OS (negative control) and p14ARF transfected IMR90 cells. Antibody: Affinity purified rabbit anti-p14ARF (Cat. No. A300-342A) used for WB at 1 µg/ml. Detection: Chemiluminescence.
Detection of human p14ARF by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF (Cat. No. A300-342A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB