Antibody was affinity purified using an epitope specific to MAD2 immobilized on solid support. The epitope recognized by A300-301A maps to a region between residue 150 and the C-terminus (residue 205) of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HSMAD2, MAD2, MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2 mitotic arrest deficient-like 1, MAD2-like protein 1, mitotic arrest deficient 2-like protein 1, mitotic arrest deficient, yeast, homolog-like 1, mitotic spindle assembly checkpoin
between 150 and C-term
2 - 8°C
Detection of human and mouse MAD2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MAD2 antibody A300-301A (lot A300-301A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MAD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2 antibody A300-301A (lot A300-301A-3) used for IP at 6 µg/mg lysate. MAD2 was also immunoprecipitated by a previous lot of this antibody (lot A300-301A-2) and rabbit anti-MAD2 antibody A300-300A. For blotting immunoprecipitated MAD2, A300-301A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.