Antibody was affinity purified using an epitope specific to FUS immobilized on solid support. The epitope recognized by A300-294A maps to a region between residue 500 and the C-terminus (residue 526) of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
75 kDa DNA-pairing protein, ALS6, ETM4, FUS/ERG chimeric protein, FUS/ERG fusion protein, FUS1, fused in sarcoma, fusion gene in myxoid liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, oncogene FUS, oncogene TLS, POMP75,
between 500 and C-term
2 - 8°C
Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-FUS antibody A300-294A (lot A300-294A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. FUS was also immunoprecipitated by a previous lot (lot A300-294A-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).