www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-FUS Antibody, Affinity Purified

Catalogue number:
A300-294A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to FUS immobilized on solid support. The epitope recognized by A300-294A maps to a region between residue 500 and the C-terminus (residue 526) of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

75 kDa DNA-pairing protein, ALS6, ETM4, FUS/ERG chimeric protein, FUS/ERG fusion protein, FUS1, fused in sarcoma, fusion gene in myxoid liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, oncogene FUS, oncogene TLS, POMP75,

Species Reactivity:

Human, Mouse

Immunogen:

between 500 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human FUS by western blot a

Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-FUS antibody A300-294A (lot A300-294A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. FUS was also immunoprecipitated by a previous lot (lot A300-294A-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).

Detection of human and mouse FUS by immu

Detection of human and mouse FUS by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS (Cat. No. A300-294A Lot2) used at a dilution of 1:5,000 (0.2µg/ml) and 1:1,000 (1µg/ml). Detection: DAB