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Rabbit anti-FUS Antibody, Affinity Purified - A300-294A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-FUS Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-294A
Name:
Rabbit anti-FUS Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to FUS immobilized on solid support. The epitope recognized by A300-294A maps to a region between residue 500 and the C-terminus (residue 526) of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
75 kDa DNA-pairing protein, ALS6, ETM4, FUS/ERG chimeric protein, FUS/ERG fusion protein, FUS1, fused in sarcoma, fusion gene in myxoid liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, oncogene FUS, oncogene TLS, POMP75,
Species Reactivity:
Human, Mouse
Immunogen:
Between 500 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-FUS antibody A300-294A (lot A300-294A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. FUS was also immunoprecipitated by a previous lot (lot A300-294A-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).

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Detection of human and mouse FUS by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS (Cat. No. A300-294A Lot2) used at a dilution of 1:5,000 (0.2µg/ml) and 1:1,000 (1µg/ml). Detection: DAB

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