Rabbit anti-SMAR1/BANP Antibody, Affinity Purified - A300-279A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to SMAR1/BANP immobilized on solid support. The epitope recognized by A300-279A maps to a region between residue 425 and the C-terminus (residue 469) of human BTG3 associated nuclear protein using the numbering given in TrEMBL entry Q96GJ7 (GeneID 54971). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human and mouse SMAR1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody A300-279A (lot A300-279A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody A300-279A (lot A300-279A-3) used for IP at 6 µg per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (lot A300-279A-2). For blotting immunoprecipitated SMAR1, A300-279A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of mouse SMAR1 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. A300-279A Lot 2 used at a dilution of 1:5,000 (0.2µg/ml) (left) and Cat. No. A300-279A Lot3 used at a dilution of 1:5,000 (0.2µg/ml) (right). Detection: DAB
Detection of human SMAR1 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. A300-279A Lot 2 used at a dilution of 1:5,000 (0.2µg/ml) (left) and Cat. No. A300-279A Lot3 used at a dilution of 1:5,000 (0.2µg/ml) (right). Detection: DAB
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