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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-PCNA Antibody, Affinity Purified
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Rabbit anti-PCNA Antibody, Affinity Purified

Catalogue number:
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A300-277A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µg
Product is available in:
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£468.00
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Estimated delivery Wed 5 Nov
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Alternative Names:

proliferating cell nuclear antigen; PCNA; DNA polymerase delta auxiliary protein; ATLD2; cyclin

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human and mouse PCNA by wes
Detection of human and mouse PCNA by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody A300-277A (lot A300-277A-1) used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PCNA by western blot
Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 µg for IP; 30 µg for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody BL1351 (Cat. No. A300-277A) used at 0.1 µg/ml for WB. B. PCNA was immunoprecipitated using BL1350 (Cat. No. A300-276A), BL1351 or a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using a mixture of BL1350 and BL1351 (each at 1 µg/ml). Detection: Chemiluminescence with an exposure times as indicated (A) or 5 seconds (B).