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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-PCNA Antibody, Affinity Purified
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Rabbit anti-PCNA Antibody, Affinity Purified

Catalogue number:
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A300-276A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00
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Estimated delivery Fri 26 Apr
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Applications:

WB, IP, IHC, Flow Cyt

Antibody Host:

Rabbit

Target:

PCNA

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 75 and 125

Alternative Names:

proliferating cell nuclear antigen; PCNA; DNA polymerase delta auxiliary protein; ATLD2; cyclin

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Product Description:

Rabbit anti-PCNA Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse PCNA by wes
Detection of human and mouse PCNA by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody A300-276A (lot A300-276A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of PCNA Versus DNA Content. As
Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X10^6 cells were stained with 0.03 µg anti-PCNA [A300-276A]. Secondary detection was performed with FITC conjugated goat F(ab')2 anti-rabbit antibody [A120-114F], and DNA stained with PI.
Detection of human PCNA by immunohistoch
Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA (Cat. No. A300-276A Lot1) used at a dilution of 1:10,000 (0.1µg/ml). Detection: DAB
Detection of human PCNA by western blot
Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 µg for IP; 30 µg for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody BL1350 (Cat. No. A300-276A) used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using BL1350, BL1351 (Cat. No. A300-277A) or a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using a mixture of BL1350 and BL1351 (each at 1 µg/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).
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