Antibody was affinity purified using an epitope specific to 53BP1 immobilized on solid support. The epitope recognized by A300-273A maps to a region between residues 1925 and the C-terminus (residue 1972) of human tumor protein p53 binding protein 1 using the numbering given in entry NP_005648.1 (GeneID 7158). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
53BP1, p202, p53-binding protein 1, p53BP1, TDRD30, TP53-binding protein 1, tumor protein 53-binding protein, 1, tumor suppressor p53-binding protein 1
between 1925 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse 53BP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-53BP1 antibody A300-273A (lot A300-273A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.