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Rabbit anti-Claspin Antibody, Affinity Purified

Catalogue number:
A300-267A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Claspin immobilized on solid support. The epitope recognized by A300-267A maps to a region between residues 900 and 950 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

claspin, claspin homolog, hClaspin

Species Reactivity:

Human

Immunogen:

between 900 and 950

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Claspin by western bl

Detection of human Claspin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Claspin antibody A300-267A (lot number A300-267A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Claspin was also immunoprecipitated by a previous lot (lot number A300-267A-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).