Antibody was affinity purified using an epitope specific to EMSY immobilized on solid support. The epitope recognized by A300-253A maps to a region between residue 1250 and the C-terminus (residue 1322) of human EMSY chromosome 11 open reading frame 30 using the numbering given in TrEMBL entry Q8TE50 (GeneID 56946). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BRCA2-interacting transcriptional repressor EMSY, C11orf30, GL002, protein EMSY
between 1250 and C-term
2 - 8°C
Detection of human EMSY by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody A300-253A (lot A300-253A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody A300-253A (lot A300-253A-2) used for IP at 6 µg/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot A300-253A-1) and rabbit anti-EMSY antibody BL1049. For blotting immunoprecipitated EMSY, A300-253A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.