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Rabbit anti-Matriptase/ST14 Antibody, Affinity Purified

Catalogue number:
A300-221A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Matriptase/ST14 immobilized on solid support. The epitope recognized by A300-221A maps to a region between residues 800 and the C-terminus (residue 855) of human Membrane-type serine protease 1 using the numbering given in SwissProt entry Q9Y5Y6 (GeneID 6768). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

ARCI11, HAI, Matriptase, membrane-type serine protease 1, MTSP1, MT-SP1, prostamin, PRSS14, serine protease 14, serine protease TADG-15, SNC19, suppression of tumorigenicity 14 (colon carcinoma), suppression of tumorigenicity 14 (colon carcinoma, matripta

Species Reactivity:

Human

Immunogen:

between 800 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Matriptase/ST14 by we

Detection of human Matriptase/ST14 by western blot. Samples: Whole cell lysate (50 µg) from MCF-7, OVCAR-4 [OV], SW620 [SW], RPMI-8266 [RPMI], and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Matriptase/ST14 antibody A300-221A (lot A300-221A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Matriptase/ST14 by we

Detection of human Matriptase/ST14 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Matriptase/ST14 antibody A300-221A (lot A300-221A-2) used for IP at 6 µg per reaction. Matriptase/ST14 was also immunoprecipitated by Rabbit anti-Matriptase/ST14 antibodies BL10867, BL10868 and BL10869. For blotting immunoprecipitated Matriptase/ST14, A300-221A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.