Antibody was affinity purified using an epitope specific to BRD8 immobilized on solid support. The epitope recognized by A300-220A maps to a region found within isoform 2 between residues 225 and 275 of human Bromodomain containing 8 using the numbering given in Swiss-Prot entry Q9H0E9.2 (GeneID 10902). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bromodomain-containing protein 8, p120, Skeletal muscle abundant protein, skeletal muscle abundant protein 2, SMAP, SMAP2, thyroid hormone receptor coactivating protein of 120 kDa, trCP120
between 225 and 275 of isoform 2
2 - 8°C
Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract ( 0.5 or 1 mg per IP reaction; 20% of IP loaded ) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody A300-220A (lot A300-220A-2) used for IP at 6 µg per reaction. BRD8 was also immunoprecipitated by a previous lot of this antibody (lot A300-220A-1). For blotting immunoprecipitated BRD8, A300-220A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.