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Rabbit anti-BRD8 Antibody, Affinity Purified - A300-220A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BRD8 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-220A
Name:
Rabbit anti-BRD8 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BRD8 immobilized on solid support. The epitope recognized by A300-220A maps to a region found within isoform 2 between residues 225 and 275 of human Bromodomain containing 8 using the numbering given in Swiss-Prot entry Q9H0E9.2 (GeneID 10902).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
bromodomain-containing protein 8, p120, Skeletal muscle abundant protein, skeletal muscle abundant protein 2, SMAP, SMAP2, thyroid hormone receptor coactivating protein of 120 kDa, trCP120
Species Reactivity:
Human
Immunogen:
Between 225 and 275 of isoform 2
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human BRD8 by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody A300-220A (lot A300-220A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract ( 0.5 or 1 mg per IP reaction; 20% of IP loaded ) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody A300-220A (lot A300-220A-2) used for IP at 6 µg per reaction. BRD8 was also immunoprecipitated by a previous lot of this antibody (lot A300-220A-1). For blotting immunoprecipitated BRD8, A300-220A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human BRD8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BRD8 (Cat. No. A300-220A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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