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Rabbit anti-BRD8 Antibody, Affinity Purified

Catalogue number:
A300-219A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to BRD8 immobilized on solid support. The epitope recognized by A300-219A maps to a region between residues 25 and 75 of human Bromodomain containing 8 using the numbering given in Uniprot entry Q9H0E9.2 (GeneID 10902). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

bromodomain-containing protein 8, p120, Skeletal muscle abundant protein, skeletal muscle abundant protein 2, SMAP, SMAP2, thyroid hormone receptor coactivating protein of 120 kDa, trCP120

Species Reactivity:

Human

Immunogen:

between 25 and 75 in Uniprot entry Q9H0E9.2)

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human BRD8 by western blot.

Detection of human BRD8 by western blot. Samples: Nuclear extract (50 and 15 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-BRD8 antibody A300-219A (lot A300-219A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human BRD8 by western blot

Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody A300-219A (lot A300-219A-2) used for IP at 6 µg per reaction. BRD8 was also immunoprecipitated by rabbit anti-BRD8 antibody A300-220A. For blotting immunoprecipitated BRD8, A300-219A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.