Antibody was affinity purified using an epitope specific to BRD8 immobilized on solid support. The epitope recognized by A300-219A maps to a region between residues 25 and 75 of human Bromodomain containing 8 using the numbering given in Uniprot entry Q9H0E9.2 (GeneID 10902). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bromodomain-containing protein 8, p120, Skeletal muscle abundant protein, skeletal muscle abundant protein 2, SMAP, SMAP2, thyroid hormone receptor coactivating protein of 120 kDa, trCP120
between 25 and 75 in Uniprot entry Q9H0E9.2)
2 - 8°C
Detection of human BRD8 by western blot. Samples: Nuclear extract (50 and 15 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-BRD8 antibody A300-219A (lot A300-219A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody A300-219A (lot A300-219A-2) used for IP at 6 µg per reaction. BRD8 was also immunoprecipitated by rabbit anti-BRD8 antibody A300-220A. For blotting immunoprecipitated BRD8, A300-219A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.