Antibody was affinity purified using an epitope specific to CKII alpha immobilized on solid support. The epitope recognized by A300-197A maps to a region between residues 300 and 350 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
casein kinase 2, alpha 1 polypeptide, casein kinase II alpha 1 polypeptide pseudogene, casein kinase II alpha 1 subunit, casein kinase II subunit alpha, CK II alpha, CK2 catalytic subunit alpha, CK2A1, Cka1, Cka2, CKII, OCNDS, protein kinase CK2
between 300 and 350
2 - 8°C
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Detection of human and mouse CKII alpha by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody A300-197A (lot A300-197A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.