Rabbit anti-CKII alpha Antibody, Affinity Purified - A300-197A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to CKII alpha immobilized on solid support. The epitope recognized by A300-197A maps to a region between residues 300 and 350 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human and mouse CKII alpha by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody A300-197A (lot A300-197A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CKII Alpha by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha (Cat. No. A300-197A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of mouse CKII Alpha by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha (Cat. No. A300-197A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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