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Rabbit anti-PELP1/MNAR Antibody, Affinity Purified - A300-180A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PELP1/MNAR Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-180A
Name:
Rabbit anti-PELP1/MNAR Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to PELP1/MNAR immobilized on solid support. The epitope recognized by A300-180A maps to a region between residues 1000 and 1050 of human proline-, glutamic acid-, leucine-rich protein 1/ modulator of nongenomic activity of estrogen receptor using the numbering given in entry AAN41255.1 (GeneID 27043).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
MNAR, modulator of non-genomic activity of estrogen receptor, P160, proline and glutamic acid rich nuclear protein, proline-, glutamic acid- and leucine-rich protein 1, transcription factor HMX3
Species Reactivity:
Human, Mouse
Immunogen:
Between 1000 and 1050
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human PELP1/MNAR by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody A300-180A (lot A300-180A-3) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody A300-180A (lot A300-180A-3) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human PELP1/MNAR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PELP1/MNAR antibody A300-180A (lot A300-180A-3) used for IP at 6 µg per reaction. PELP1/MNAR was also immunoprecipitated by a previous lot of this antibody (lot A300-180A-2) and rabbit anti-PELP1/MNAR antibody A300-876A For blotting immunoprecipitated PELP1/MNAR, A300-180A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human PELP1/MNAR by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibodies: Affinity purified rabbit anti-PELP1/MNAR Cat. No. A300-180A Lot2 used at a dilution of 1:2,500 (0.4µg/ml) (left) and Cat. No. A300-180A Lot3 used at a dilution of 1:5,000 (0.2µg/ml) (right). Detection: DAB

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Detection of mouse PELP1/MNAR by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-PELP1/MNAR Cat. No. A300-180A Lot2 used at a dilution of 1:2,500 (0.4µg/ml) (left) and Cat. No. A300-180A Lot3 used at a dilution of 1:5,000 (0.2µg/ml) (right). Detection: DAB

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