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Goat anti-Chk1 Antibody, Affinity Purified - A300-162A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Goat anti-Chk1 Antibody, Affinity Purified
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Size: 100 µg
£309.00
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Catalogue number:
A300-162A
Name:
Goat anti-Chk1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Chk1 immobilized on solid support. The epitope recognized by A300-162A maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
cell cycle checkpoint kinase, Checkpoint kinase-1, Checkpoint, S. pombe, homolog of, 1, CHK1, CHK1 checkpoint homolog, Chk1-S, serine/threonine-protein kinase Chk1
Species Reactivity:
Human
Immunogen:
Between 250 and 300
Storage Temp:
2 - 8°C
Antibody Host:
Goat
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Chk1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody A300-162A (lot A300-162A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Chk1 by western blot and immunoprecipitation. Sample: Whole Cell lysate (50 µg for WB, 500 µg for IP) from HeLa cells. Antibodies: Affinity purified goat anti-Chk1 (Cat No. A300-162A) used as indicated. immunoprecipitatesd material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.

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