Antibody was affinity purified using an epitope specific to Chk1 immobilized on solid support. The epitope recognized by A300-162A maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cell cycle checkpoint kinase, Checkpoint kinase-1, Checkpoint, S. pombe, homolog of, 1, CHK1, CHK1 checkpoint homolog, Chk1-S, serine/threonine-protein kinase Chk1
between 250 and 300
2 - 8°C
Detection of human Chk1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody A300-162A (lot A300-162A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Chk1 by western blot and immunoprecipitation. Sample: Whole Cell lysate (50 µg for WB, 500 µg for IP) from HeLa cells. Antibodies: Affinity purified goat anti-Chk1 (Cat No. A300-162A) used as indicated. immunoprecipitatesd material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.